Fig. 2

Immunoblotting of B-T0, BBAC-T0, B-T4, BBAC-T4 and A. a Representative example of bevacizumab detected after immunoblotting on a PVDF membrane. 10 ng (1), 25 ng (2) or 50 ng (3) were loaded per lane. Two distinct bands of 25 and 55 kDa were detected, corresponding to the light and heavy chains of the IgG molecule. (b/c) Densitometry analysis of the heavy (b) and light (c) chain (n = 2)