Table 3 Canine mast cell tumour-specific primer sequences
From: Establishment and characterisation of a novel canine mast cell tumour cell line (C18)
Primer name | Primer (5’ to 3’) | Product size (Bps) | Accession No. or reference |
|---|---|---|---|
C-kit | F: CACCGACCCAGGATTTGTCA | 230 | NM_001003181.1 |
| Â | R: GGACCAGCGTATCATTGCCT | Â | Â |
Tryptase | F: ACTACACACCCGAGAATGGG | 243 | NM_001097555.2 |
| Â | R: GGTGGTATTGCACGTCACAC | Â | Â |
Chymase | F: AGTTTGGAAGGAGTGGGAGTG | 282 | U89607.1 |
| Â | R: ACCTAGTGTGGAGAGGTCAGG | Â | Â |
FcεRIα | F: GTCATCAGATACCTTGAAACCTACA | 294 | NM_001110766.2 |
| Â | R: ACGTTGGCAGAGGCTTGAA | Â | Â |
FcεRIβ | F: TTCTGGGGGTAACACAAAT | 402 | (7) |
| Â | R: TGCAAAACCCCAGAATAGT | Â | Â |
RPS5 | F: TCACTGGTGAGAACCCCCT | 141 | XM_533568.4 |
| Â | R: CCTGATTCACACGGCGTAG | Â | Â |