Fig. 4

Immunohistochemical analysis of AMPK (A, B) and PLIN2 (C, D) in paraffin sections. The expression of p-AMPK and PLIN2 in healthy controls and pyometra affected uterine tissue samples was examined using immunohistochemistry (IHC) at x20 (100 μm) magnification with Aperio ScanScopev12.4.6 digital scanning analysis of slides; (E) IHC staining score (* p < 0.05; ** p < 0.01)