Fig. 2
From: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector
Recombinant pDEV-GAstV ORF2 construction. pDEV-EF1 (shown in the dotted box (A and B)) were constructed and detailed in our prior publication [18]. A. Homologous recombination was used to introduce a mini-F BAC vector (pHA2) that allows for large circular DNA to be maintained in E. coli into the intergenic region between UL15B and UL18 in a DEV vaccine strain. B. Two-step Red-mediated recombination (en passant) was used to substitute the PCMV promoter controlling GFP expression in pDEV-vac for the pEF1 gene. C-D. The recombinant BAC clone pDEV-kan.GAstV ORF2 was constructed by first inserting PCMV-GAstV ORF2-BGH-pA-Kan expression cassette into the US7 and US8 intergenic spacer in pDEV-EF1(C) and pDEV-kan.GAstV ORF2 (D) were obtained by deleting Kan gene from pDEV-kan.GAstV ORF2 by 2nd homologous recombinant