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Table 1 Primers used in this study

From: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

Primer name

Primer Sequence

pDEV vac-in-s

5‘-TACTAATTTAAGTGTGCAGCCTGGTTAACTGTATTAT

GCGCGGAGCGATGTACGGGCCAGATA-3‘

pDEV vac-in-as

5‘-TCCGTAGTCTGGCCGGCAGTATGTTGGTGTTTAGTACT

CCAAACCCATAGAGCCCACCGCATCCCC-3‘

JD-F

5‘-CTACCACAAGCGTCATCAACCA-3‘

JD-R

5‘-TGTCCATTACCAAATCCGAAAA-3‘

  1. a Sequences shown in bold are homologous to intergenic insertion site sequences for DEV US7 and US8, introducing homologous recombinant arms
  2. b Italicized sequences are primers (F and R shown in Fig. 1) homologous to two terminus of pCMV-GAstV ORF2-Kan expression cassette in pEP-BGH-GAstV ORF2
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BMC Veterinary Research

ISSN: 1746-6148

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