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Table 3 Impact of arginine with or without Olive leaf extract supplementations on the antioxidant potential of chicken breast meat during frozen storage

From: Combined modulatory effects of dietary arginine and olive leaf phenolic extract on growth performance and immune functions of broiler chickens, and meat antioxidant potential during frozen storage

Parameter

 

Experimental groups

p-value

Control

G

G + OLEǀ

G + OLEǀǀ

G + OLEǀǀǀ

FRAP, 3 h

0.18 ± 0.03d

0.23 ± 0.03c

0.25 ± 0.02c

0.31 ± 0.01b

0.37 ± 0.01a

< 0.001

FRAP, 4 weeks

0.26 ± 0.002c

0.31 ± 0.003c

0.37 ± 0.002b

0.43 ± 0.003a

0.47 ± 0.015a

< 0.001

DPPH, 3 h

5.89 ± 0.02e

6.20 ± 0.02d

6.30 ± 0.04c

6.90 ± 0.04b

7.40 ± 0.04a

< 0.001

DPPH, 4 weeks

6.30 ± 0.07e

6.70 ± 0.08d

6.90 ± 0.03c

7.15 ± 0.05b

7.57 ± 0.06a

< 0.001

ABTS, 3 h

2.40 ± 0.03e

3.30 ± 0.04d

3.60 ± 0.03c

4.30 ± 0.07b

4.50 ± 0.06a

< 0.001

ABTS, 4 weeks

5.40 ± 0.05c

5.70 ± 0.2c

6.03 ± 0.59c

7.67 ± 0.04b

8.4 ± 0.16a

< 0.001

TBARS, 3 h

0.19 ± 0.006a

0.17 ± 0.016a

0.12 ± 0.01b

0.09 ± 0.01b

0.043 ± 0.02c

< 0.001

TBARS, 4 weeks

0.80 ± 0.03a

0.71 ± 0.04b

0.63 ± 0.03c

0.58 ± 0.03c

0.51 ± 0.02d

< 0.001

Total flavonoids (µg/g)

74.50 ± 1.1d

102.75 ± 3.2c

105.10 ± 1.1c

128.73 ± 1.40b

138.40 ± 1.80a

< 0.001

Total phenolic compounds (µg/g)

85.40 ± 0.68e

127.30 ± 0.95d

143.60 ± 4.90c

150.90 ± 1.20b

164.60 ± 4.50a

< 0.001

SOD (U/mL)

52.25 ± 5.10e

63.95 ± 3.60d

78.68 ± 1.60c

112.90 ± 3.10b

124.66 ± 4.30a

< 0.001

GPX (µmol/mg)

304.38 ± 1.70d

332.45 ± 6.30c

345.65 ± 3.60b

364.00 ± 5.50a

368.48 ± 24.30a

< 0.001

CAT (U/L)

31.92 ± 1.90d

47.23 ± 2.50c

58.04 ± 0.79b

81.42 ± 6.90a

86.91 ± 5.30a

< 0.001

T-AOC (U/mg protein)

7.6 ± 0.57d

9.2 ± 0.08c

10.2 ± 0.71b

11 ± 0.06b

12.7 ± 0.07a

< 0.001

  1. FRAP: ferric reducing antioxidant power; DPPH, 1,1-diphenyl-2-picrylhydrazyl radical; ABTS: 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid); TBARS: thiobarbituric acid reactive substances; SOD: superoxide dismutase; GPX: glutathione peroxidase; CAT: Catalase; T-AOC: total antioxidative capacity. Control: chicks fed basal diets without any supplementations, G: chicks fed control diets fortified with 1.5 g/kg arginine (G), G + OLEǀ: chicks fed control diets fortified with 1.5 g/kg G plus 0.25% olive leaf extract (OLE), G + OLEǀǀ: chicks fed control diets fortified with 1.5 g/kg G plus 0.5% OLE, and G + OLEǀǀǀ: chicks fed control diets fortified with 1.5 g/kg G plus 1% OLE. The data are displayed as means ± standard error of the mean (SEM). a, b,c, d,e various superscript letters in a row imply statistical significance (p < 0.05)