Table 5 Primers’ sequences used for quantitative reverse transcription polymerase chain reaction analysis
Specificity/ Target gene | Primer sequence (5’-3’) | Accession No. | |
|---|---|---|---|
House keeping | |||
GAPDH | F: GGTGGTGCTAGCGTGTTA | NM205518 | |
R: CCCTCCACAATGCCAA | |||
Antioxidant enzymes | |||
NQO1 | F: TCGCCGAGCAGAAGAAGATTGAAG | NM_001277620.1 | |
R: CGGTGGTGAGTGACAGCATGG | |||
HO-1 | F: AAGAGCCAGGAGAACGGTCA | NM_205344 | |
R: AAGAGCCAGGAGAACGGTCA | |||
Nrf2 | F: GAGCCCATGGCCTTTCCTAT | NM_001007858.1 | |
R: CACAGAGGCCCTGACTCAAA | |||
GPX-1 | F: AACCAATTCGGGCACCAG | HM590226 | |
R: CCGTTCACCTCGCACTTCTC | |||
SOD-1 | F: GGCAATGTGACTGCAAAGGG | NM_205064.1 | |
R: CCCCTCTACCCAGGTCATCA | |||
CAT | F: GGGGAGCTGTTTACTGCAAG | NM_001031215.2 | |
R: GGGGAGCTGTTTACTGCAAG | |||
PRDX-1 | F: CTGCTGGAGTGCGATTGT | NM_001271932.1 | |
R: GCTGTGGCAGTAAAATCAGGG | |||
XOR | F: GTGTCGGTGTACAGGATACAGAC | NM_205127.1 | |
R: CCTTACTATGACAGCATCCAGTG | |||
HMOX1 | F: ACACCCGCTATTTGGGAGAC | NM_205344.1 | |
R: GAACTTGGTGGCGTTGGAGA | |||
Muscle development | |||
mTOR | F: CATGTCAGGCACTGTGTCTATTCTC | XM_417614.5 | |
R: CTTTCGCCCTTGTTTCTTCACT | |||
MyoG | F: GGAGAAGCGGAGGCTGAAG | NM_204184.1 | |
R: GCAGAGTGCTGCGTTTCAGA | |||
MyoD | F: CAGCAGCTACTACACGGAATCA | NM_204214.2 | |
R: GGAAATCCTCTCCACAATGCTT | |||
MSTN | F: ATGCAGATCGCGGTTGATC | NM_001001461.1 | |
R: GCGTTCTCTGTGGGCTGACT | |||
Immune related genes | |||
IgA | F: ACCACGGCTCTGACTGTACC | S40610.1 | |
R: CGATGGTCTCCTTCACATCA | |||
IgM | F: AGGAGACAGGACTGGAATGCACAA | XM_025906584.1 | |
R: GGAGGCAGTATAGGTATCATCCTC | |||
IgG | F: GAGGGAAGGGAAGAGTTACAGC | X07174 | |
R: GTGTTCCTGTAGACGCTCTTGC | |||