Fig. 2

Schematic depiction of Pasteurella multocida strain P-1059 filamentous hemagglutinin B1 ( fhaB1 ) gene. A). The arrows indicate the priming sites used to amplify 2,867 bp fragment of fhaB1 by PCR with the primer pair, fhaB1F/fhaB1R (Table 1). B). After digestion with EcoRV, 1,090 bp was deleted from fhaB1. The deleted fragment was joined to the Ts origin and kanamycin resistance element to create the replacement plasmid used to generate the ΔfhaB1 deletion. C). The wild-type parent (W) and ΔfhaB1 mutant (M) strains of P. multocida were analyzed by PCR using the primer pair fhaB1F/fhaB1R