Table 1 Oligonucleotides used in this study
Oligonucleotide | Sequence | Target | Product |
|---|---|---|---|
fhaB1F | 5`-CTCAACCAGCGCTTAACTCGC-3` | primer used to generate fhaB1 fragment | fhaB1 bp 131–3003 |
fhaB1R | 5`-GTTTTCTTGTCTAACTGTACACTGTTA-3` | primer used to generate fhaB1 fragment | |
fhaB1F | 5`-CTCAACCAGCGCTTAACTCGC-3` | forward primer RT PCR analysis set 1 | fhaB1 bp 131–317 |
fhaB1RT1 | 5`- AATTCAGGTTTTCTT TGTACCACTG-3` | reverse primer RT PCR analysisset 1 | |
fhaB1F | 5`-CTCAACCAGCGCTTAACTCGC-3` | forward primer RT PCR analysis set 2 | fhaB1 bp 131–700 |
fhaB1RT2 | 5`-CAATGCCATTTTGGTTCGCAA − 3` | reverse primer RT PCR analysisset 2 | |
fhaB1F | 5`-CTCAACCAGCGCTTAACTCGC-3` | forward primer RT PCR analysis set 3 | fhaB1 bp 131–926 |
fhaB1RT3 | 5`-GTGATATCGGTTTCAGAATTTCCT-3` | reverse primer RT PCR analysisset 3 | |
Ts ori F | 5`-GATCCCTTTTTCTGTAATCT-3` | forward primer Ts origin of replication | Tsorigin1160 bp |
Ts ori R | 5’-GATCATAGGCTCAATTCTCG-3` | reverse primer Ts origin of replication | |
KanR F | 5`-ATGAGCCATATTCAACGG-3` | forward primer Tn903 kan resistance element | 860 bp |
KanR R | 5`-TCAGAAAAACTCATCGAGCATC-3` | reverse primer Tn903 kan resistance element |