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Table 2 Real-time PCR analyses of fhaB1 transcripts

From: Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults

Primer set

Template RNA

Ct

Tm (°C)

1

P-1059 wild-type log-phase

16.58

80.05

1

P-1059 wild-type stationary

18.07

80.16

1

P-1059 ΔfhaB1 mutant

17.5

80.34

2

P-1059 wild-type log-phase

18.82

81.27

2

P-1059 wild-type stationary

20.9

81.14

2

P-1059 ΔfhaB1 mutant

19.73

81.08

3

P-1059 wild-type log-phase

18.58

81.37

3

P-1059 wild-type stationary

19.54

81.61

3

P-1059 ΔfhaB1 mutant

28.79

63.46

  1. Ct = cycle threshold, Tm = melting temperature
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BMC Veterinary Research

ISSN: 1746-6148

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