Table 2 The primes for standard plasmids construction and phylogenetic analysis
Purpose | Primer | Sequence (5’-3’) | Length | Gene |
|---|---|---|---|---|
Standard plasmids constructions | SIV-F | AGTCTTCTAACCGAGGTCGAAACGT | 978 bp | M |
SIV-R | TTACTCCAGCTCTATGTTGACAAAA | |||
PRRSV-F | ATACCAACTTCCTTCTGGACACTAA | 1191 bp | M, N | |
PRRSV-R | CATGGTTCTCGCCAATTAAA | |||
PCV2-F | CAGCACCTCGGCAGCACCTCAGCAG | 1725 bp | Rep, Cap | |
PCV2-R | CGGTTTCAGCGATGACGTATCCAAG | |||
Phylogenetic analysis | PRRSV-F | GTTTTAGCCTGTCTTTTTGCC | 704 bp | GP5 |
PRRSV-R | AAGGTGCTTTTGGCGTTTTC | |||
PCV2-F | CAGCACCTCGGCAGCACCTCAGCAG | 1700 bp | Rep, Cap | |
PCV2-R | CGGTTTCAGCGATGACGTATCCAAG |