Table 3 Triple fluorescence quantitative PCR reaction system
Reagent | Volume |
|---|---|
Premix Ex Taq(Probe qPCR)(2X) | 10 µL |
ROX Reference Dye II | 0.2 µL |
SIV-F and SIV-R (10µM) | 0.8 µL (0.4µM) |
SIV-Pro (10µM) | 0.4 µL (0.2µM) |
PRRSV-F and PRRSV-R (10µM) | 0.8 µL (0.4µM) |
PRRSV- Pro (10µM) | 0.6 µL (0.3µM) |
PCV2-F and PCV2-R (10µM) | 0.8 µL (0.4µM) |
PCV2- Pro (10µM) | 0.4 µL (0.2µM) |
Template | 2 µL |
Enzyme-free water | Up to 20 µL |