Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2

Table 4 Specificity of the multiplex real-time PCR assay

Sample Type	Controls	Multiplex Real-Time PCR in the Study (Ct Value)
Sample Type	Controls	PRRSV	PCV2	SIV
Lung	PRRSV	26.214	-	-
Serum	PCV2	-	26.950	-
Nasal swab	SIV	-	-	30.110
Lung	CSFV	-	-	-
Lung	PRV	-	-	-
Bacterial fluid	HPS	-	-	-
Bacterial fluid	SS	-	-	-
Porcine alveolar macrophages	PAM	-	-	-

ISSN: 1746-6148