Table 5 Sensitivity of triplex qPCR assay
Pathogen | Concentrations (Copies/µL) | Repeat times | Positive number | Positive rate | 95% positive rate |
|---|---|---|---|---|---|
SIV | 100 | 23 | 23 | 100% | >95% |
10 | 23 | 12 | 52% | <95% | |
1 | 23 | 0 | 0% | <95% | |
PRRSV | 100 | 23 | 23 | 100% | >95% |
10 | 23 | 22 | 96% | >95% | |
1 | 23 | 0 | 0% | <95% | |
PCV2 | 100 | 23 | 23 | 100% | >95% |
10 | 23 | 23 | 100% | >95% | |
1 | 23 | 0 | 0% | <95% |