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Table 1 Genus-specific 16S rRNA gene primers used for molecular identification, along with their corresponding amplicon sizes and annealing temperatures

From: Emergence of dual drug-resistant strongylids in goats: first phenotypic and genotypic evidence from Ratchaburi Province, central Thailand

Genetic marker and genus

Primer name and sequence (5’– 3’)

Annealing temperature

Amplicon size

16S Haemonchus

16S Hae-F: CATATTTRATCCAGAWG

45 °C

116 bp

16S Hae-R: CGTTAAATTAAATAAWAG

  

16S Trichostrongylus

16S Tricho-F: CTAGGGTAGAATATTATT

41 °C

173 bp

16S Tricho-R: AAAGAAGAACAGTCTTAAT

  

16S Oesophagostomum

16S Oeso-F: CTTCGGAAATTCTTTTTTGG

51 °C

205 bp

16S Oeso-R: CTTCTCCCTCTTTAACAAAC

  
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BMC Veterinary Research

ISSN: 1746-6148

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