BMC Veterinary Research

Table 1 Primers used for qRT-PCR validation

From: Comparative study of the effects of baicalin and probenecid on microRNA expression profiles in porcine aortic vascular endothelial cells infected by Glaesserella parasuis

Gene	Nucleotide Sequence (5’−3’)		Tm (°C)
ssc-miRNA-127	Forward	CGTCGGATCCGTCTGAGC	59.1
ssc-miRNA-127	Reverse	AGTGCAGGGTCCGAGGTATT	59.1
ssc-miRNA-1842	Forward	GCGTTGGCTCTGCGAGG	58.9
ssc-miRNA-1842	Reverse	AGTGCAGGGTCCGAGGTATT	58.9
ssc-miRNA-9810-3p	Forward	GCGAGCACGCGGCCA	59.6
ssc-miRNA-9810-3p	Reverse	AGTGCAGGGTCCGAGGTATT	59.6
ssc-miR-1296-5p	Forward	TTAGGGCCCTGGCTCCATCTCC	64
ssc-miR-1296-5p	Reverse	AGTGCAGGGTCCGAGGTATT	64
ssc-miR-27b-5p	Forward	AGAGCTTAGCTGATTGGTGAACA	59
ssc-miR-27b-5p	Reverse	AGTGCAGGGTCCGAGGTATT	59
U6	Forward	CTCGCTTCGGCAGCACA	60
U6	Reverse	AACGCTTCACGAATTTGCGT	60
HBEGF	Forward	GGCAGACCTGGACCTTTTGA	59.7
HBEGF	Reverse	CACGTACTTGCACTCTCCGT	59.7
TOP2A	Forward	TCAAACGAAATGACAAGCGAG	60
TOP2A	Reverse	AACTGACCAATGGGCTGTAAGA	60
β-actin	Forward	TGCGGGACATCAAGGAGAAG	60
β-actin	Reverse	AGTTGAAGGTGGTCTCGTGG	60

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ISSN: 1746-6148

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