Fig. 3

Molecular characterization of pigeon adenovirus. (A) Agarose gel showing the results of PCR with primers specific for the hexon and fiber-2 genes of PiAdV-1. S1–S6: pigeon liver supernatants collected from Beijing, Hebei, Xinjiang, Shanxi, Shaanxi, and, Inner Mongolia were subjected to hexon gene amplification, and the band is located at approximately 3064 bp; S7–S12: pigeon liver supernatants collected from Beijing, Hebei, Xinjiang, Shanxi, Shaanxi, and, Inner Mongolia were subjected to fiber-2 gene amplification, and the band is located at approximately 2678 bp; C: negative control; M: DL5000 bp ladder marker. For consistency in presentation, ‘S1’ and the negative control were cropped, reordered, and stitched with ‘S2-S6’. Similarly, ‘S7’ and the negative control were cropped, reordered, and stitched with ‘S8-S12’. The full-length gel for the left panel is presented in Supplementary Fig. 1, and for the right panel in Supplementary Fig. 2. (B) Phylogenetic tree based on alignment of sequenced hexon gene PCR products with reference avian adenovirus strains. (C) Phylogenetic tree based on alignment of sequenced fiber-2 gene PCR products with reference avian adenovirus strains. Trees were generated using the neighbor-joining method, coupled with the Kimura 2-parameter model and bootstrap analysis of 1000 replicates. In (B) and (C), the five colors (red, green, magenta, blue and light yellow) of the outer ring sequentially represent the five species of Aviadenoviruses: DAdV, FAdV, TAdV, PiAdV, and GoAdV, while the different background colors in the inner circle represent distinct clades. Trees show the name and GenBank accession number of each isolate, and the six strains highlighted in red font were obtained in this study