Fig. 6

Box and whisker chart presenting concentrations of (A) progesterone (P4), (B) free estrogens (E2), and (C) conjugated estrogens (E2S) in cell culture media from equine granulosa cells collected by OPU and treated for 24 h either with 100 ng/ml IGF1 or with 20 µM H89 (PKA blocker) in the absence or presence of cortisol in the following dosages: 50 ng/ml, 100 ng/ml and 150 ng/ml, respectively. Experiments (n = 4 biological replicates) were conducted using pooled granulosa cells obtained from the ovaries of four to six mares for each experiment. Significant differences and the respective P values are presented in each graph. Different superscripts indicate significant differences (P < 0.05) within each cortisol concentration, whereas bars indicate significant differences among different cortisol concentrations. *P < 0.05