Fig. 1

Bioinformatics prediction of sheep ICOS gene. A Similarity comparison of sheep ICOS gene. B Phylogenetic tree construction of sheep ICOS gene; C Hydrophilicity /hydrophobicity prediction of sheep ICOS protein; D Hydrophilicity and antigenic epitope prediction of sheep ICOS protein (a. Hydrophilicity prediction; b. Antigenic epitope prediction); E Transmembrane region prediction of sheep ICOS protein (red line indicates transmembrane region; blue line indicates intramembrane region; purple line Indicates extramembrane region); F Signal peptide prediction of sheep ICOS protein (Sec/SPI n. N-terminal region of signal peptide; Sec/SPI h. Central hydrophobic region of the signal peptide; Sec/SPI c. C-terminal region of signal peptide). G Prediction of potential phosphorylation sites of sheep ICOS protein. H Prediction of potential glycosylation sites of sheep ICOS protein