Fig. 4

Expression, expression form, detection of purification results and Western blotting identification of recombinant proteins of sheep ICOS. A detection of sheep ICOS recombinant protein expression form (M. Protein molecular weight marker; 1. Pre-induction product of recombinant bacteria; 2. Post-induction product of recombinant bacteria; 3. Supernatant of induced product; 4. Precipitation of induced product); B Purification results of sheep ICOS recombinant protein (M. Protein molecular weight maker; 1. Purified recombinant protein). C Purification results of sheep ICOS recombinant Protein (M. Protein molecular weight marker; 1. Purified recombinant protein; 2. Extracted total natural protein)