Fig. 4

Effect of the different treatments on haemolysis. Relative haemoglobin percentage of sheep RBCs treated for 4 h in vitro at 37 °C with different concentrations of PUFAs-ω3 and PUFAs-ω6 (25, 100, 200, and 250 µg/mL), and using the ratio ω3/ω6 of 1:2 (100/200 µg/mL), 1:4 (25/100 µg/mL), 1:10 (25/250 µg/mL). Untreated RBCs after 4 h at 37 °C represent the control group (CTRL). Uppercase letters indicate significant differences, between groups of RBCs supplemented with different concentrations of PUFAs Uppercase letters indicate significant statistical differences between groups of RBCs supplemented with different concentrations of PUFAs (red bars indicate groups with added ω3. Blue bars indicate groups with added ω6. Blue/red bars indicate groups with added ω3/ω6 ratio. Black bar indicated CTRL): p < 0.05 (one-way ANOVA)