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Table 1 Roche Diagnostics ELISA kit provides detailed performance data for each analyte

From: Short-term effects of a synbiotic diet on thyroid and sex hormones in Sarabi Dogs

Analyte

Sensitivity (Lower Limit of Detection, LLOD)

Specificity (Cross-reactivity)

TSH

 ~ 0.005 µIU/mL

No significant cross-reactivity with FSH, LH, hCG

Free T4

 ~ 0.3 pmol/L

Minimal interference with binding proteins

Total T3

 ~ 0.3 nmol/L

 < 1% cross-reactivity with T4

LH/FSH

 ~ 0.1 IU/L

LH assay specificity: < 0.1% with hCG/FSH

Testosterone

 ~ 0.025 nmol/L (males)

High specificity for testosterone (low DHT cross-reactivity)