Fig. 1

Phase-contrast images of neonatal bovine intestinal organoid culture. (A) Phase-contrast images of growing organoids embedded in ECM droplets from the initial establishment for five sections of neonatal bovine gut. Organoids from passages 0 or 1 are shown. (B) Phase-contrast images of growing organoids successfully resuscitated after over 300 days of cryopreservation, demonstrating the capacity for long-term storage and revival. (C) Organoids resuscitated from cryopreservation-maintained growth and preserved their morphology over more than 15 serial passages. All images in (A), (B), and (C) were captured between days 4 and 12 of culture. Scale bar = 100 μm