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Table 2 Summary of viability across stages of neonatal bovine intestinal organoid culture by intestinal segment

From: From in vitro development to accessible luminal interface of neonatal bovine-derived intestinal organoids

Intestinal segment

Establishment (P0)

Resuscitation following cryopreservation

Serial passaging following cryopreservation

Duodenum

5/5

4/5

3/4

Jejunum

5/5

3/5

1/3

Ileum

5/5

4/5

4/4

Colon

5/5

4/5

4/4

Rectum

5/5

5/5

5/5

  1. Culture success for each gut section from the five donor animals. The numerator represents the successful cultivation of at least one growing organoid, evaluated by daily monitoring using a phase-contrast microscope. The denominator indicates the total number of donor samples from which cultures were attempted