Comprehensive study of ticks and tick-borne diseases in dogs in Nepal: molecular identification, risk analysis and hematological alterations

Table 1 Primer sets for PCR amplification of canine tick-borne diseases

Pathogens	Primer Name	Primers (5’-> 3’)	Target gene	Amplicon size (bp)	Reference
Babesia	BTF1	GGC TCA TTA CAA CAG TTA TAG	18srRNA	930	[55]
	BTR1	CCC AAA GAC TTT GAT TTC TCT C		930
	BTF2	CCG TGC TAA TTG TAG GGC TAA TAC		800
	BTR2	GGA CTA CGA CGG TAT CTG ATC G		800
Hepatozoon canis	HEP-F	ATA CAT GAG CAA AAT CTC AAC	18srRNA	666	[56]
Hepatozoon canis	HEP-R	CTT ATT ATT CCA TGC TGC AG	18srRNA	666	[56]
Ehrlichia canis	ECA	AAC ACA TGC AAG TCG AAC GGA	16srRNA	400	[57]
Ehrlichia canis	HE3	TAT AGG TAC CGT CAT TAT CTT CCC TAT	16srRNA	400	[57]
Borrelia burgdorferi	BFL1	GCTCAATATAACCAAATGCACATG	16srRNA	422	[58]
Borrelia burgdorferi	BFL2	CAAGTCTATTTTGGAAAGCACCTAA	16srRNA	422	[58]
Anaplasma platys	Platys	GAT TTT TGT CGT AGC TTG CTA TG	16srRNA	678	[54]
Anaplasma platys	EHR16SR	TAG CAC TCA TCG TTT ACA GC	16srRNA	678	[54]

ISSN: 1746-6148