Prevalence and antimicrobial resistance profile of Listeria spp. isolated from raw fish

BMC Veterinary Research

Table 2 Primers used for PCR-Based identification of Listeria spp

Species	Gene	Primer	Sequences (5’-3’)	PCR product size (bp)	Anneling temterature	Reference
Listeria spp.	prs	prs-F	GCTGAAGAGATTGCGAAAGAAG	370	58°	[42]
Listeria spp.	prs	prs-R	CAAAGAAACCTTGGATTTGCGG	370	58°	[42]
Listeria spp.	spp	spp-F	CTGGCACGGACTTCCACTTAC	116	55°	[43]
Listeria spp.	spp	spp-R	TTTGAGCACCGATGATGATTT	116	55°	[43]
L. monocytogenes	Glycosyl hydrolase	lm-F	GTTCGTCGGTCCGTGGTA	583	55°	[44]
L. monocytogenes	Glycosyl hydrolase	lm-R	TTGGCAAGCAAGCAGTTCA	583	55°	[44]
L. ivanovii	namA	iv-F	CGAATTCCTTATTCACTTGAGC	463	53°	[45]
L. ivanovii	namA	iv-R	GGTGCTGCGAACTTAACTCA	463	53°	[45]
L. innocua	lin0464	in-F	CGCATTTATCGCCAAAACTC	749	54°	[46]
L. innocua	lin0464	in-R	TCGTGACATAGACGCGATTG	749	54°	[46]
L. seeligeri	lmo0333	se-F	GTACCTGCTGGGAGTACATA	673	52°	[47]
L. seeligeri	lmo0333	se-R	CTGTCTCCATATCCGTACAG	673	52°	[47]
L. welshimeri	scrA	we-F	CGTGGCACAATAGCAATCTG	281	52°	[47]
L. welshimeri	scrA	we-R	GACATGCCTGCTGAACTAGA	281	52°	[47]
L. grayi	Oxidoreductase	gr-F	GCGGATAAAGGTGTTCGGGTCAA	201	61°	[47]
L. grayi	Oxidoreductase	gr-R	ATTTGCTATCGTCCGAGGCTAGG	201	61°	[47]

ISSN: 1746-6148