Table 3 Primer pairs used for amplification of antibiotic resistance gene in Listeria spp. Isolates
From: Prevalence and antimicrobial resistance profile of Listeria spp. isolated from raw fish
Group of antibiotics | Gene | Primer | Sequences (5’-3’) | PCR product size (bp) | Annealing temperature | Reference |
|---|---|---|---|---|---|---|
Β-lactamas | penA | penA-F | ATCGAACAGGCGACGATGTC | 500 | 52° | [48] |
penA-R | GATTAAGACGGTGTTTTACGG | |||||
ampC | ampC-F | TTCTATCAAMACTGGCARCC | 550 | 50° | [49] | |
ampC-R | CCYTTTTATGTACCCAYGA | |||||
blaTEM | bla-F | TTTCGTGTCGCCCTTATTCC | 690 | 60° | [49] | |
bla-R | CCGGCTCCAGATTTATCAGC | |||||
blaZ | blaZ-F | ACTTCAACACCTGCTGCTTTC | 490 | 60° | [49] | |
blaZ-R | TGACCACTTTTATCAGCAACC | |||||
Sulfonamides | sulI | sulI-F | GTGACGGTGTTCGGCATTCT | 779 | 68° | [50] |
sulI-R | TCCGAGAAGGTGATTGCGCT | |||||
sulII | sulII-F | CGGCATCGTCAACATAACCT | 721 | 66° | [50] | |
sulII-R | TGTGCGGATGAAGTCAGCTC | |||||
Macrolides | ermB | ermB-F | GAAAAGGTACTCAACCAAATA | 639 | 52° | [48] |
ermB-R | AGTAACGGTACTTAAATTGTTTAC | |||||
ermA | ermA-F | AACACCCTGAACCCAAGGGACG | 420 | 62° | [48] | |
ermA-R | CTTCACATCCGGATTCGCTCGA | |||||
ereB | ereB-F | AGAAATGGAGGTTCATACTTACCA | 546 | 53° | [48] | |
ereB-R | CATATAATCATCACCAATGGCA | |||||
Glycopeptides | vanA | vanA-F | CATGACGTATCGGTAAAATC | 885 | 52° | [48] |
vanA-R | ACCGGGCAGRGTATTGAC | |||||
vanB | vanB-F | CATGATGTGTCGGTAAAATC | 882 | 52° | [48] | |
vanB-R | ACCGGGCAGRGTATTGAC |