Fig. 5

Phylogenetic tree based on 16S rRNA sequences of four sequenced Enterobacter isolates. The phylogenetic tree was inferred via the neighbor‒joining method [28], and the optimal tree is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches [29]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances (number of nucleotide substitutions per site) were computed via the Kimura-2-parameter method [30]. All ambiguous positions were removed for each sequence pair. There was a total of 1519 positions in the final dataset. Evolutionary analyses were conducted in MEGA11 [31]