Fig. 1

Linearity assessment of serially diluted CRCoV synthetic oligonucleotide detection using RT-qPCR and RT-dPCR. (a) RT-qPCR standard curve for CRCoV synthetic oligonucleotide, displaying the correlation between the quantification cycle (Cq) values (Y axis) against the logarithm of the CRCoV synthetic oligonucleotide concentration (X axis). (b) RT-dPCR standard curve for CRCoV synthetic oligonucleotide, showing the correlation by plotting the logarithm of the absolute concentration (Y axis) against the logarithm of the CRCoV synthetic oligonucleotide concentration (X axis)