Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples

Table 1 Repeatability and reproducibility analysis of RT-qPCR

Calculated concentration of CRCoV synthetic oligonucleotide (copies/µL)	Intra-assay variation (repeatability)			Inter-assay variation (reproducibility)
	Mean (Cq)	SD	CV (%)	Mean (Cq)	SD	CV (%)
3.65 × 10⁶	20.48	0.46	2.25	21.07	0.47	2.23
3.65 × 10⁵	24.90	0.30	1.20	25.09	0.31	1.23
3.65 × 10⁴	29.87	0.09	0.30	29.39	0.42	1.43

Cq = Quantification cycle; SD = Standard deviation; CV = Coefficient of variation

ISSN: 1746-6148