Table 2 Repeatability and reproducibility analysis of RT-dPCR
Calculated concentration of CRCoV synthetic oligonucleotide (copies/µL) | Intra-assay variation (repeatability) | Inter-assay variation (reproducibility) | ||||
|---|---|---|---|---|---|---|
Mean (copies/µL) | SD | CV (%) | Mean (copies/µL) | SD | CV (%) | |
3.65 × 104 | 13883.73 | 92.75 | 0.67 | 12021.87 | 2399.04 | 19.46 |
3.65 × 103 | 1203.20 | 24.60 | 2.00 | 1129.07 | 221.51 | 19.62 |
3.65 × 102 | 91.12 | 5.43 | 5.95 | 74.59 | 9.54 | 12.79 |