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Table 3 Comparison of detection range between RT-qPCR and RT-dPCR using serial dilutions of CRCoV standard positive control

From: Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples

Calculated concentration of CRCoV synthetic oligonucleotide (copies/µL)

RT-qPCR

RT-dPCR

3.65 × 108

3.65 × 107

3.65 × 106

3.65 × 105

3.65 × 104

3.65 × 103

3.65 × 102

1.83 × 102

3.65 × 101

3.65 × 100

1.83 × 100

3.65 × 10− 1

+

+

+

+

+

+

+

+

-

-

-

-

NA

NA

NA

Overload

+

+

+

+

+

+

+

-

  1. + = Positive; - = Negative; NA = Not available; Overload = The number of template molecules in a partition exceeds the optimal limit, compromising quantification accuracy