Encapsulated phytogenic oils enhance in vitro rumen fermentation and reduce methane emissions

BMC Veterinary Research

Table 2 Effect of microencapsulated black soldier fly-based protein extract (BSF) mixed with garlic oil extract (GOE) and nanoemulsified riceberry rice bran oil (nRBRBOE) ratio supplementation on kinetic of in vitro gas production and nutrient degradability

T¹	mBSF-GOE to nRBRBOE ratios, (mg)	Gas kinetics²				Gas (96 h) mL/0.2 g DM	IVDMD (% DM)
T¹	mBSF-GOE to nRBRBOE ratios, (mg)	a	b	c	a + b	Gas (96 h) mL/0.2 g DM	12 h	24 h
T1	0:0	-3.3^b	96.1^a	0.029^b	92.8^a	89.9^a	29.0	34.2^b
T2	6:0	0.1^b	98.5^a	0.029^b	98.6^a	95.6^a	27.2	37.6^a
T3	4:2	0.9^b	51.0^b	0.067^a	51.9^b	53.4^b	26.7	39.9^a
T4	2:4	6.4^a	96.2^a	0.029^b	102.6^a	99.8^a	25.9	39.6^a
T5	0:6	7.9^a	95.1^a	0.029^b	103.0^a	98.5^a	29.6	38.8^a
SEM		0.41	1.95	0.08	1.83	1.59	0.79	0.80
Orthogonal polynomials
Linear		< 0.01	0.04	0.01	0.37	0.45	0.347	< 0.01
Quadratic		0.28	< 0.01	< 0.01	< 0.01	< 0.01	0.826	0.03
Cubic		0.11	< 0.01	< 0.01	< 0.01	< 0.01	0.883	0.38

¹T, each treatment in this experiment; ² Gas production kinetics; a, the gas production from the immediately soluble fraction (mL); b, the gas production from the insoluble fraction (mL); c, the gas production rate constant for the insoluble fraction (mL/h); a + b, the potential extent of gas production (mL); IVDMD, in vitro dry matter degradability; SEM, standard error of mean; ^a−b Means with different superscripts within a column are significantly different (p < 0.05); treatments are expressed as mean and values are calculated from a minimum of three replicates

ISSN: 1746-6148