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First report of multidrug-resistant and pathogenic Plesiomonas shigelloides from endangered crested ibis (Nipponia nippon)

BMC Veterinary Research volume 21, Article number: 299 (2025) Cite this article

Abstract

Plesiomonas shigelloides (P. shigelloides) is a gram-negative, thermotolerant, motile, and pleomorphic microorganism that acts as an opportunistic pathogen, capable of causing abscesses, enteric infections, and even sepsis in humans and other animals, thereby posing a threat to human and animal health worldwide. The crested ibis (Nipponia nippon) is a globally endangered avian species with a limited population and is vulnerable for various infections. To gain a better understanding of the prevalence of P. shigelloides in the endangered crested ibis, fecal samples from the crested ibis at the Xiazhu Lake Crested Ibis Breeding Research Center, China were collected and analyzed. The results confirmed that 45.6% (36/79) of the fecal samples tested positive for P. shigelloides. Antibiotic susceptibility testing showed that 27.8% (10/36) of P. shigelloides isolates were multidrug-resistant, with resistance to ampicillin and amikacin being 100% and 77.8%, respectively. Subsequently, ten representative isolates were subjected to whole genome sequencing, and resistance and virulence genes were analyzed. The evolutionary tree of the genomes show that these strains have different origins, with one strain closely related to a strain from human. Finally, the pathogenicity experiment demonstrated that several strains exhibit pathogenicity in both mice and waterfowl, with two strains displaying lethal pathogenicity in mice. To the best of our knowledge, this is the first report of multidrug-resistant and pathogenic P. shigelloides isolated from the crested ibis, suggesting a potential threat to this endangered species and broader public health implications.

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Introduction

Crested ibis (Nipponia nippon) is a flagship species of the endangered wildlife conservation in the world, which is known as an “Oriental gem” in China [1, 2]. Wild populations were almost extinct in the historically widespread areas throughout Eastern Russia, Korea, Japan, to central China. The number of populations decreased sharply due to the deterioration of the environment and other factors [3, 4]. After ornithologists saw the wild crested ibis for the last time in 1964, no one ever discovered them again over a decade. Until 1981, a small, single wild population of crested ibises (consisting of two pairs and three nestlings) was discovered in Yang County, Shaanxi Province, China, by Chinese scientists. Since then, China has initiated and continued the protection of the crested ibis to this very day, making a significant contribution to the maintenance of wildlife biodiversity [4, 5].

Plesiomonas shigelloides (P. shigelloides) is an opportunistic pathogen that can cause abscesses, enteric infections and even sepsis in humans and other animals, posing a threat to human and animal health, worldwide [6, 7]. P. shigelloides is widely distributed and could be isolated from the water environment and various hosts, such as fish, shellfish, crustaceans, waterfowls, mammals, amphibians, reptiles, birds, and other vertebrates [8,9,10,11]. Over the past several years, the infection with P. shigelloides has attracted increasing attention, particularly in children, the elderly, and those with impaired immune systems. Drinking or even being exposed to untreated water increases the likelihood of suffering from diarrhea caused by P. shigelloides [12, 13]. Therefore, it needs to paid attention for the public health brought about by cross-infection of P. shigelloides.

Although the population of the crested ibis has significantly increased compared to the past, we still need to maintain ongoing attention to their health. With the expansion of artificial breeding programs, bacterial diseases have become one of the limiting factors in the conservation efforts for the crested ibis. There have been quite a few reports of waterfowls carrying P. shigelloides with or without symptoms [14,15,16]. The scarlet ibis (Eudocimus ruber) belongs to the same family of ibises as the crested ibis. In 2016, Castelo-Branco et al. discovered P. shigelloides in captive scarlet ibises and their habitats, but no follow-up research was conducted [17]. The overlap between the foraging environment of the crested ibis and the habitat of P. shigelloides heightens the risk of infection in these birds.

To better understand the prevalence and evaluate potential risks of P. shigelloides in the crested ibis’s population, we chose the only population-gathering site of the crested ibis in southern China, which is located at Xiazhu Lake, Deqing County, Zhejiang Province. Fresh feces of crested ibises were collected. Additionally, we investigated the isolation, molecular characteristics, antibiotic susceptibility, and pathogenicity of the isolated P. shigelloides strains. We then assessed the prevalence of P. shigelloides in the southern breeding grounds of the crested ibis’s population, analyzed the whole-genome characteristics of representative strains, and evaluated their antibiotic resistance and pathogenicity profiles. We anticipate that these findings will provide a foundation for the prevention of diseases affecting the crested ibis and address the potential public health risks posed by P. shigelloides.

Materials and methods

Ethical statement

All experiments were conducted in accordance with the guidelines and regulations outlined by the Management and Use of Laboratory Animals of Zhejiang Province and complied with China’s existing laws and regulations for biological research. We only collected feces from the cages of the crested ibises and did not perform any procedures on these birds, minimizing the impact on them.

The animals’ experiments were conducted in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals approved by the State Council of the People’s Republic of China. The protocol received approval from the Institutional Animal Care and Use Committee of Zhejiang A&F University (Permit Number: ZAFUAC2023001).

Sample collection

The fresh fecal samples were collected from the crested ibis in Xiazhu Lake Crested Ibis Breeding Research Center, the largest crested ibis breeding base in China, located in Deqing County, Zhejiang Province. At the time of sampling, January 2021, there were 394 of the captive-bred crested ibis, and none of the birds had used antibiotics within 6 months before the study nor showed any evident signs of disease. The crested ibis are known for their timidity and susceptibility to being easily startled. To minimize stress, fecal samples were collected from their enclosures with minimal disturbance. In total, 79 fresh fecal samples were collected with maximum effort. The samples were then transported to the laboratory within 2 h on the day of collection.

Isolation and biochemical identification of P. shigelloides

The bacterial strains were preliminarily identified according to Bergey’s Manual of Systematic Bacteriology and Common Bacterial System Identification Manual. The P. shigelloides strains were identified by using bacterial isolation and culture, morphological and biochemical identification. Colonies isolation or purification using brain-heart infusion (BHI), Salmonella-Shigella (SS) or sheep blood agar plates. In brief, round, colorless, semi-transparent, smooth-surfaced, moist small colonies on SS agar plates of gram-negative bacilli were selected for biochemical identification, including the lactose, sucrose, maltose, raffinose, sorbitol, hydrogen sulfide, lysine decarboxylase, ornithine decarboxylase and amino acid decarboxylase control.

Sequence analysis of 16S rDNA

The isolates were seeded into BHI broth medium and cultured overnight. Total genomic DNA was isolated from the liquid culture using FastPure Bacteria DNA Isolation Kit (Vazyme) according to the manufacturer’s instructions. The full length of 16S rDNA was amplified by PCR with the universal primers, 27 F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGA CTT-3′) [18]. Amplification products were recovered and sequenced by Tsingke Biotechnology (China). The obtained 16S rRNA sequences were analyzed by the NCBI BLAST retrieval system for sequence homology. Then, a phylogenetic tree of the 16S rRNA gene sequences was constructed by comparing the sequences of the isolates with those of other bacterial species retrieved from GenBank [9, 10], using the neighbor-joining method in MEGA software v11.0.10. A bootstrap analysis with 1000 replicates was used to estimate the reliability of each tree topology.

Molecular typing of P. shigelloides by enterobacterial repetitive intergenic consensus (ERIC)-PCR

ERIC-PCR possessed potentially superior resolution merits in intra-typing of P. shigelloides [19]. It was performed on different P. shigelloides strains with the primers, ERIC-F (5’-ATGTAAGCTCCTGGGGATTCAC-3’) and ERIC-R (5’-AAGTAAG TGACTGGGGTGAGCG-3’). After PCR reaction was performed, the amplification products were placed in 1% agarose gel for electrophoresis and photographed on a UV transilluminator for further analysis by GelJ software v_2_2 refer to previous research [19].

Antibiotic susceptibility testing

The sensitivity of the isolated strains to antibiotics was investigated using the Kirby–Bauer disc diffusion method, following the procedure detailed by the Clinical and Laboratory Standards Institute (CLSI) [20]. Susceptibility was categorized as susceptible, intermediate or resistant by measuring the diameter of inhibition zone according to the criteria stipulated by the CLSI. P. shigelloides strains were subjected to antimicrobial susceptibility to relevant antibiotics including ampicillin, cefotaxime, cefoperazone, meropenem, gentamicin, amikacin, ciprofloxacin, enrofloxacin, norfloxacin, florfenicol, polymyxin B, and tetracycline. E. coli ATCC25922 served as quality control.

Whole-genome sequencing and analysis

Ten representative strains based on results of ERIC-PCR and antibiotics susceptibility testing were selected for the second-generation sequencing, which was commissioned from Institute of Microbiology, Chinese Academy of Sciences. Utilize the CGE website (Center for Genomic Epidemiology) to conduct SNP-based phylogenetic analysis on the whole-genome sequences of ten selected strains of P. shigelloides, as well as on thirty-one genomic sequences of P. shigelloides isolated from different sources (including human, fish, birds, water, food and unknown) downloaded from the PATRIC database (Table S1). Then, the phylogenomic analysis of the strains was constructed. Meanwhile, the results of whole-genome sequencing were compared with the Virulence Factor Database (VFDB, http://www.mgc.ac.cn/VFs) and the Comprehensive Antibiotic Research Database (CARD, http://arpcard.mcmaster.ca) respectively, for the knowledge of the virulence genes and antimicrobial resistance genes carried by these strains.

Detection of antibiotic resistance genes

Based on the results of whole genome sequencing, resistance genes, blaTEM, aac(6’)-Ib3, aac(6’)-Ib-cr, mph(A), arr-2, tet(A), qacEΔ1, dfrA1 and sulI were detected by PCR and sequencing for the P. shigelloides strains isolated from crested ibis. Primers of the resistance genes referred to the published literatures [21,22,23,24].

Pathogenicity of P. shigelloides in mice and ducks

The pathogenicity of ten representative P. shigelloides strains was confirmed by conducting experimental challenges on six weeks old ICR mice and one-week-old Muscovy ducks, to evaluate the disease-causing potential of isolated strains both in mammals and waterfowls. Firstly, the strains were grown in BHI broth medium and cultured with agitation at 37℃ for 12 h. The strains were washed twice with 10 mmol/L phosphate-buffered saline (PBS) and then adjusting the optical density (OD) 600 value to 1 × 1010 CFU/mL corresponding to the number of bacteria and then enumerating the bacteria with plate counting method. For mice experiment, the ICR mice were separated into PBS control group and bacteria-inoculated groups. The mice were challenged intraperitoneally with 1 × 109 CFU and control group were inoculated intraperitoneally with sterile PBS. Continuous monitoring was conducted on weight loss, clinical symptoms, and behaviors of each mouse for 14 d.p.i. Two strains (P. shigelloides-18 and P. shigelloides 36) exhibiting significant weight loss were further evaluated for survival rates. Post-mortem examinations were promptly performed on deceased mice to observe pathological changes, and bacterial isolation and identification were conducted from the organs.

For the infection experiment on ducks, one-week-old, clinically healthy Muscovy ducks were used in this study. For each strain, the inoculated groups (three ducks for each group, n = 3) were inoculated intraperitoneally with of the indicated bacteria with 1 × 109 CFU. The control group were inoculated intraperitoneally with equal volume of PBS. The birds were also observed for clinical symptoms, morbidity and mortality for up to 14 days.

Results

Isolation, identification and typing of P. shigelloides

In January 2021, the fecal samples were collected from the crested ibises in Xiazhu Lake crested ibis Breeding Research Center, which is currently the largest crested ibis breeding base in China, located in Deqing County, Zhejiang Province, China. A total of 79 fresh fecal samples were randomly collected from this population. The bacterium grew well on BHI medium, SS medium and sheep blood medium. The colonies were round, beige, opaque, smooth, moist, and slightly raised but with neat edges in BHI medium (Fig. 1A and B). Gram staining results were microscopically observed. The bacterial cells stained red and appeared as short rods, which were arranged singly or in pairs, indicating that the strain was gram-negative (Figure 1C). Meanwhile, biochemical identification results demonstrated that the bacterium could hydrolyze maltose, glucose, and myo-inositol, but not lactose, sucrose, raffinose, or sorbitol. Additionally, positive results were obtained for lysine decarboxylase and ornithine decarboxylase tests (Figure 1D).

Fig. 1
figure 1

Isolation, identification, and biochemical characteristics of P. shigelloides. Colonial morphology of P. shigelloides isolate in BHI agar plate (A), SS agar plate (B) and morphological characteristics (C) observed under 100×oil immersion lens with Gram staining. (D) Biochemical identification results of all P. shigelloides isolates. Biochemical projects a-i refers to lactose, sucrose, maltose, raffinose, sorbitol, hydrogen sulfide, lysine decarboxylase, ornithine decarboxylase and amino acid decarboxylase control

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To further characterize the isolates, 16S rRNA genes were amplified and sequenced. Homology analysis using NCBI BLAST revealed a similarity of over 99% between these strains and P. shigelloides. Based on 16S rRNA-based phylogeny, these strains were determined to be P. shigelloides (Figs. 2A). Therefore, based on the morphological, physiological, biochemical and molecular characteristics, these isolates were identified as P. shigelloides. Overall, 45.6% (36/79) of the fecal samples were positive and totally 36 strains of P. shigelloides were isolated, these isolates were named as P. shigelloides 1–36.

Fig. 2
figure 2

Identification and typing of P. shigelloides. (A) Phylogenetic tree based on partial 16S rRNA gene sequences and constructed in MEGA software v11.0.10 by using the neighbor-joining method. A bootstrap analysis with 1000 replicates was used to estimate the reliability of each tree topology. (B) Molecular typing of P. shigelloides by ERIC-PCR

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Followed by ERIC-PCR typing, these isolates can be divided into six categories, namely ERIC-I to ERIC-VI types (Fig. 2B). Among them, ERIC-VI type is the most prevalent, containing nine isolates. Followed by ERIC-IV and ERIC-V types, each contains eight isolates.

Results of antibiotic susceptibility testing

In order to further understand the antibiotic resistance patterns of these isolates, susceptibility of all isolates to different antibiotics were determined using the disk diffusion method on Mueller-Hilton agar plates according to recommendations of CLSI. The results show that the highest prevalence of resistance was observed for ampicillin (36/36 = 100%), followed by amikacin (28/36 = 77.8%) and ciprofloxacin (11/36 = 30.6%). Enrofloxacin, norfloxacin and florfenicol resistance was only detected in one isolate (P. shigelloides-21, P. shigelloides-29 and P. shigelloides-33, respectively) (Fig. 3A, Table S2). Specifically, 27.8% (10/36) of the isolates were multidrug resistant (resistant to three or more categories of antimicrobials) (Table S2).

Fig. 3
figure 3

Antibiotic susceptibility testing and detection of antibiotic resistance genes of all the 36 strains of P. shigelloides isolated from crested ibis. Percentage distribution of antibiotics susceptibility testing (A) and percentage of antibiotic resistance genes (B) of all the 36 P. shigelloides strains isolated from crested ibis

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Genome sequencing and phylogenetic analysis of the representative strains

According to the results of molecular typing and drug resistance, ten representative strains were selected for next-generation sequencing. The genome assembly results showed that the number of raw reads produced by ten representative strains of P. shigelloides ranged from 8,943,568 to 15,067,496 and the genome length were ranged from 3,614,060 bp to 3,809,779 bp, and the average content of GC was 51.8% (ranged from 51.59 to 51.95%) (Table 1).

Table 1 Genome assembly results of 10 representative strains of P. shigelloides
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Subsequently, the phylogenomic analysis of P. shigelloides based on SNP (Parsnp) was performed, which contained ten representative strains in this study and thirty-one strains downloaded from PATRIC database (Table S1). The results showed that the sequences of these strains exhibited a quite distinct difference in overall similarity, yet it is still can be seen that the ten representative strains possibly originated from different sources (Fig. 4A). 70.0% (7/10) of the representative strains of P. shigelloides isolated from the crested ibis belong to a distinct phylogenetic branch that does not include strains from other sources. These strains are clustered with a foodborne isolate, P. shigelloides strain Colony20 from Thailand, indicating a degree of species specificity among certain strains of P. shigelloides, including those from the crested ibis. In contrast, the remaining three P. shigelloides isolates in this study are associated with different sources. Specifically, P. shigelloides-15 is clustered with P. shigelloides strain P5462, isolated from a penguin in Hong Kong. P. shigelloides-36 is clustered with P. shigelloides strain MS-17-188, derived from a catfish in eastern Mississippi, USA. Notably, P. shigelloides-18 is clustered with P. shigelloides strain NCTC10364, isolated from human feces in Japan.

Fig. 4
figure 4

Phylogenetic tree based on the genomes of P. shigelloides and analysis of virulence related and antibiotic resistance genes. (A) Ten representative strains were selected for next-generation sequencing and the phylogenomic analysis of P. shigelloides basing on SNP (Parsnp) were performed. The isolates in this study were labeled in red. (B) Whole-genome sequences were compared with the Virulence Factor Database (VFDB) and the Comprehensive Antibiotic Research Database (CARD) respectively. The distribution of virulence genes and drug resistance genes of the ten isolates are represented by a heat map. The positives were labeled in red and the negatives were represented by blanks

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Analysis of virulence related and antibiotic resistance genes in sequenced genomes

Furthermore, the virulence-related and antibiotic resistance genes of these stains were analyzed. Whole-genome sequences were submitted and compared using the VFDB and CARD platforms, respectively. The results revealed that a total of 45 virulence genes were predicted in ten representative strains of P. shigelloides, including 16 core virulence genes. All the strains carried genes of flagellar P-ring protein (flgI), flagellar switch protein (fliG), general secretory pathway protein D (gspD SS206), capsular polysaccharide (wecA) and double-component bacterial regulation (cheV). There were nine strains carried the genes of flagellar switch protein (fliN) and general secretory pathway protein E (epsE), and eight strains carried sigma-54-dependent transcriptional activator (flrA). All representative strains in this study lacked genes related to the cytotoxic outer membrane protein ComP. Notably, all strains harbored over twenty virulence genes, with P. shigelloides-18 and P. shigelloides-36 possessing the highest number of virulence genes, at twenty-four and twenty-six, respectively (Fig. 4B). Notably, some unique virulence genes, such as hcp, flhA.1, wbtK, pilT.1, glnA1.1 and vasK/icmF only distributed in P. shigelloides-36.

Meanwhile, nine common antibiotic resistant genes were detected in the ten strains of P. shigelloides as well, including blaTEM, mph(A), tet(A), arr-2, aac(6’)-Ib-cr, aac(6’)-Ib3, dfrA1, sulI and qacEΔ1, which were related to β-lactams, macrolides, tetracycline, rifamycin, quinolones, aminoglycosides, folic acid antagonist, sulfonamides and quaternary ammonium compounds resistance, respectively. Among them, blaTEM was distributed in all the ten strains (100%) and tet(A) was detected in six strains (60.0%). Arr-2, aac(6’)-Ib-cr, aac(6’)-Ib3, dfrA1, sulI and qacEΔ1 were positive in four strains (40.0%). Mph(A) was distributed in two strains (20.0%). Six strains were identified as carrying more than three antibiotic-resistant genes, among which four strains carried eight resistance genes (P. shigelloides-6, P. shigelloides-12, P. shigelloides-29, P. shigelloides-32) (Fig. 4B).

Furthermore, based on the results of whole genome sequencing, the above nine resistance genes were detected by PCR and sequencing for the remaining P. shigelloides strains. The prevalence of antibiotic resistance genes was shown in Fig. 3B. The prevalence of the blaTEM gene is the highest at 100%, followed by qacEΔ1 at 97.2% and sulI at 94.4%. The lowest positive rate is observed for mph(A) at 16.7%, while the carrying rates for other resistance genes range from 27.8 to 47.2%. Overall, 97.2% of the strains tested positive for more than two resistance genes.

Pathogenicity of the isolates in mice and ducks

The two tested strains (P. shigelloides-18 and P. shigelloides-36) showed pathogenicity to both mice and ducks. Ducks exhibited diarrhea on the second day after challenging, but none of the ducklings died (data not shown). Mice infected with P. shigelloides-18 and P. shigelloides-36 exhibited significant weight loss, amounting to 15% and 20%, respectively, by the third day post-infection (Fig. 5A). Notably, P. shigelloides-18 and P. shigelloides-36 resulted in case fatality rates of 33.3% and 66.7% in mice by the second day post-infection (Fig. 5B). Additionally, swelling of the spleens was observed in mice, from which P. shigelloides was subsequently recovered (data not shown).

Fig. 5
figure 5

Pathogenicity of theP. shigelloidesisolates in mice. The weight loss of mice infected by the ten representative P. shigelloides (A) and survival rate of mice infected by two strains (P. shigelloides-18 and P. shigelloides 36) (B) were monitored for 14 d.p.i. Data are presented as mean ± standard deviations (SDs)

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Discussion

Wild birds have received widespread attention as reservoirs for various pathogens, especially zoonotic pathogens [25, 26]. As a rare wild bird in China, the crested ibis has been found to be infected with E. coli, Chlamydia psittaci, Chlamydia ibidis, and Newcastle disease viruses previously [27,28,29]. P. shigelloides is an important conditional and potential pathogen for humans and animals, which could be isolated from the environment and various animals [6, 30]. In this study, P. shigelloides was the first time isolated from crested ibis’ population with a high positivity rate (about 45.6%). The genetic evolution analysis conducted in this study revealed that the P. shigelloides isolates found in the crested ibis population possibly originated from diverse sources, including humans, penguins, catfish, and various environmental settings (Fig. 4A). This finding underscores the potential for cross-regional and cross-species transmission of P. shigelloides, although the specific transmission pathways warrant further investigation. Additionally, the antibiotic resistance and virulence profiles of these P. shigelloides strains have garnered increased attention.

Antibiotic resistance has long been a threat to global public health and sustainable development, and this major scientific issue involves multiple fields [31, 32]. P. shigelloides are potential pathogens associated with intestinal diseases, and most gastrointestinal diseases caused by this bacterium do not require medical intervention [33]. However, some moderate to severe diarrhea cases often necessitate appropriate antibiotic treatment [33]. In a study of antibiotic susceptibility testing for 54 P. shigelloides strains causing diarrhea, 22.2% (12/54) of the strains were multidrug resistant, with resistance to ampicillin, tetracycline, erythromycin being 77.7%, 29.2%, and 18.5%, respectively [34]. P. shigelloides isolated from the endangered Chinese sturgeon (Acipenser sinensis) demonstrated resistance to ampicillin, penicillin, midecamycin, oxacillin, and clindamycin, while exhibiting sensitivity to cefatriaxone, piperacillin, cefoperazone, cefazolin, and ciprofloxacin [9]. Additionally, Selim et al. confirmed that P. shigelloides isolated from shellfish and aquatic samples displayed varying degrees of susceptibility to the tested antimicrobial agents, including nalidixic acid, carbenicillin, cephalothin, erythromycin, kanamycin, tetracycline, and ciprofloxacin, with kanamycin and tetracycline proving to be the most effective antibiotics against P. shigelloides, achieving effectiveness rates of 87% [35].

In this study, the results of antibiotic susceptibility testing and resistance genes identification show that all P. shigelloides bacteria are resistant to ampicillin and carry the blaTEM gene (Table S2, Fig. 3A and B). Since the breeding base for the crested ibis has never used ampicillin, these strains may be naturally resistant to ampicillin. Avison et al. reported that some P. shigelloides are naturally resistant to ampicillin, which is consistent with our findings [36]. Though a comprehensive study on Plesiomonas diarrhea revealed that 85% of the cases resolved on their own, antimicrobial treatment might be required for moderate to severe diarrhea [37]. Ciprofloxacin is one of the recommended antibiotics for treating severe dysentery-like diarrhea caused by P. shigelloides [38]. Meanwhile, aminoglycoside antibiotics, particularly amikacin, reduced the surface hydrophobicity and motility of P. shigelloides strains, making it a promising treatment option [39]. In this study, certain strains showed different levels of resistance to amikacin and ciprofloxacin, primarily due to the resistance genes aac(6’)-Ib3 and aac(6’)-Ib-cr. aac(6’)-Ib3 belong to the aac(6’) gene family coding for an aminoglycoside acetyltransferase AAC(6’) that acetylates the 6’-amino group of 4,6-disubstituted 2-deoxystreptamines such as amikacin [40]. AAC(6’)-Ib-cr, a variant of the gene for aminoglycoside acetyltransferase AAC(6’)-Ib, diminishes ciprofloxacin’s effectiveness by N-acetylating the amino nitrogen on its piperazinyl group [41]. Being one of the plasmid mediated quinolone resistance (PMQR) genes, aac(6’)-Ib-cr is frequently found in gram-negative bacteria resistant to fluroquinolones [42, 43]. Also, the possibility by the overexpression of certain efflux pumps cannot be dismissed.

Fortunately, only 27.8% of the strains exhibited multidrug resistance, with over 90.0% of the strains remaining sensitive to cefoperazone, florfenicol, and meropenem. This suggests that the antibiotic resistance of P. shigelloides in the crested ibis’ population is not severe, possibly due to the fact that crested ibis breeding centers hardly use antibiotics and strictly follows the feed hygiene standards of GB 2707, GB 2733, and GB 13,078 (Chinese national standard) for formulating crested ibis’ feed. Despite the fact that these crested ibis have rarely been given antibiotics, the isolated P. shigelloides still possess multiple antibiotic resistance genes. Given that P. shigelloides are quite often detected in water habitats and on aquatic creatures like fish, shellfish, and certain waterfowls, the P. shigelloides identified in crested ibis likely originates from these sources [8,9,10, 44]. Consequently, the antibiotic resistance genes found in these P. shigelloides isolates could be inherent [45] or acquired from commensal bacteria in aquatic environments through plasmids or horizontal gene transfer [46, 47]. As a prevalent pathogen among aquatic animals, the presence of resistance genes in P. shigelloides poses a potential threat to global public health from a “One Health” perspective [48].

The infection of P. shigelloides is complex, and many virulence genes are involved, such as hemolysin, flagellin, elastase, enterotoxin, cholera-like toxins, cytotoxic outer membrane protein (comP), and the factors related to secretion system [35, 49, 50]. Among these, the cytotoxic outer membrane protein ComP may be a major virulence factor responsible for host mortality after infection [49], while hemolysins, enterotoxins, and cholera-like toxins can cause varying degrees of damage to the host [51]. Fimbriae, capsules, and secretory system-related proteins play a significant role in promoting bacterial growth and enhancing their competitive advantages [35, 50].

In this study, mice and Muscovy ducklings were utilized as model organisms to assess the pathogenicity of representative isolates of P. shigelloides isolates. Pathogenicity assessments have demonstrated that P. shigelloides isolates exhibit relatively low pathogenic potential, which may be attributed to several factors. Firstly, the representative isolates employed in this study were deficient in the critical virulence factor, the cytotoxic outer membrane protein ComP. Additionally, given that these isolates were derived from the crested ibis, they may show reduced susceptibility to causing infections in Muscovy ducklings and mice. Due to the endangered status of the crested ibis, utilizing this species for pathogenicity assessments is not feasible. Nonetheless, it is important to note that the isolates have been observed to cause mortality in mice. Especially, P. shigelloides-18 and P. shigelloides-36 appeared to exhibit greater pathogenicity in mouse models compared to other strains. The explanation might be that P. shigelloides-18 and P. shigelloides-36 contain the highest counts of virulence genes, numbering twenty-four and twenty-six, respectively (Fig. 4B). Notably, distinct virulence genes, including hcp, flhA.1, wbtK, pilT.1, glnA1.1, and vasK/icmF, are exclusively found in P. shigelloides-36, while fliC, wecE, PSPA7, wbfU, and pliB are unique to P. shigelloides-18 (Fig. 4B). These virulence factors play a role in flagellum formation, hemolysin regulation, adherence and attachment to villi, carbohydrate metabolism, and more, potentially enhancing the strains’ virulence [35, 38, 52, 53]. Future research should prioritize enhancing health monitoring protocols for the crested ibis and evaluating the impact of P. shigelloides on the health of this species.

In summary, for the first time, we have isolated P. shigelloides from the endangered crested ibis (36/79, 45.6%). These P. shigelloides exhibited varying degrees of antibiotic resistance, with a multidrug resistance rate of 27.8%, and carried different numbers of virulence genes. The pathogenicity observed in mice and Muscovy ducks indicates that the representative P. shigelloides strains had relatively weak pathogenicity, but further investigation is still needed. Whole genomic analysis shows that P. shigelloides strains from the crested ibis exhibit phylogenetic relationships with P. shigelloides from various sources, including penguins, catfishes, environments, and even humans. This suggests that P. shigelloides from the crested ibis may disseminate across various environments and pose a potential health risk to humans. This highlights the necessity for increased surveillance of the bacteria from the crested ibis and its habitat to safeguard the health of both animals and humans.

Data availability

Data is provided within the manuscript or supplementary files. Sequence data that support the findings of this study have been deposited in the GenBank with the primary accession number JBJKNT000000000, JBJKNU000000000, JBJKNV000000000, JBJKNW000000000, JBJKNX000000000, JBJKNY000000000, JBJKNZ000000000, JBJKOA000000000, JBJKOB000000000, and JBJKOC000000000, which are also stated in the manuscript. Uncropped gel image for ERIC-PCR of Figure 2B is presented in Figure S1 in supplementary files.

References

  1. Yu F, Sun Y, Wang T, Skidmore AK, Ding C, Ye X. Linking the past and present to predict the distribution of Asian crested ibis (Nipponia nippon) under global changes. Integr Zool. 2022;17(6):1095–105.

    Article  CAS  PubMed  Google Scholar 

  2. Ran J, Wan Q, Fang S. Gut microbiota of endangered crested Ibis: establishment, diversity, and association with reproductive output. PLoS ONE. 2021;16(4):e0250075.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kim G, Jeong KC, Choi EH, Ryu SH, Lim YJ, Jun J, Lee YS, Hwang UW. The complete mitochondrial genome of an Asian crested ibis Nipponia Nippon (Pelecaniformes, Threskiornithidae) from South Korea. Mitochondrial DNA Part B. 2019;4(2):3707–8.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Yan L, Huang Z. Thirty-Five years conservation experience of crested Ibis(Nipponia nippon) and future perspectives (in Chinese). Chin J Wildl. 2021;42(3):890–6.

    Google Scholar 

  5. Yang J, Xu Z, Wan D, Wang X, Zhang X, Zhu Y, Guo J. Pollution characteristics of heavy metals, antibiotic and antibiotic resistance genes in the crested ibis and their habitat across different lifestyle and geography. Environ Res 2024, 261.

  6. Adam Y, Brezellec P, Espinosa E, Besombes A, Naquin D, Paly E, Possoz C, Dijk Ev, Barre F, Ferat J. Plesiomonas shigelloides, an atypical enterobacterales with a Vibrio-Related secondary chromosome. Genome Biol Evol. 2022;14(2):evac011.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Janda JM, Abbott SL, McIver CJ. Plesiomonas shigelloides revisited. Clin Microbiol Rev. 2016;29(2):349–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Qian Q, Chen Z, Xu J, Zhu Y, Xu W, Gao X, Jiang Q, Zhang X. Pathogenicity of Plesiomonas shigelloides causing mass mortalities of largemouth bass (Micropterus salmoides) and its induced host immune response. Fish Shellfish Immunol 2023, 132(108487).

  9. Deng D, Mu Z, Lv X, Jiang X, Zhou J, Guo H, Zhang W, Wang B, Lu YW, Du JP. H: Pathogenicity of Plesiomonas shigelloides and Citrobacter freundii isolated from the endangered Chinese sturgeon (Acipenser sinensis). Microb Pathog 2022, 173(Pt A):105818.

  10. Chen H, Zhao Y, Chen K, Wei Y, Luo H, Li Y, Liu F, Zhu Z, Hu W, Luo D. Isolation, Identification, and Investigation of Pathogenic Bacteria From Common Carp (Cyprinus carpio) Naturally Infected With Plesiomonas shigelloides. Frontiers in Immunology 2022, 13(872896).

  11. Kim K, Lee H, Kwak D. Enhanced asymptomatic systemic infection caused by Plesiomonas shigelloides in a captive Gray Wolf (Canis lupus). Veterinary Sci. 2021;8(11):280.

    Google Scholar 

  12. Clark RB, Westby GR, Spector H, Soricelli RR, Young CL. Fatal Plesiomonas shigelloides septicaemia in a splenectomised patient. J Infect. 1991;23(1):89–92.

    Article  CAS  PubMed  Google Scholar 

  13. Kelly GC, Rachmat A, Hontz RD, Sklar MJ, Tran LK, Supaprom C, Luy M, Lina S, Gregory MJ, Sopheab H, et al. Etiology and risk factors for diarrheal disease amongst rural and peri-urban populations in Cambodia, 2012–2018. PLoS ONE. 2023;18(3):e0283871.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Matsuyama R, Kuninaga N, Morimoto T, Shibano T, Sudo A, Sudo K, Asano M, Suzuki M, Asai T. Isolation and antimicrobial susceptibility of Plesiomonas shigelloides from great cormorants (Phalacrocorax carbo hanedae) in Gifu and Shiga prefectures, Japan. J Vet Med Sci. 2015;77(9):1179–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Nimmervoll H, Wenker C, Robert N, Albini S. Septicaemia caused by Edwardsiella tarda and Plesiomonas shigelloides in captive Penguin chicks. Schweizer Archiv Für Tierheilkunde. 2011;153(3):117–21.

    Article  CAS  PubMed  Google Scholar 

  16. González-Rey C, Siitonen A, Pavlova A, Ciznar I, Svenson SB, Krovacek K. Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent. Folia Microbiol. 2011;56(2):178–84.

    Article  Google Scholar 

  17. Castelo-Branco DSCM, Silva ALd, Monteiro FOB, Guedes GMM, Sales JA, Oliveira JSd M, Junior JE, Miranda SA, Sidrim JJC et al. Alencar LPd: Aeromonas and Plesiomonas species from scarlet ibis (Eudocimus ruber) and their environment: monitoring antimicrobial susceptibility and virulence. Antonie van Leeuwenhoek 2016, 110(1):33–43.

  18. Greisen K, Loeffelholz M, Purohit A, Leong D. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol. 1994;32(2):335–51.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Magyar T, Gyuris É, Ujvári B, Metzner M, Wehmann E. Genotyping of Riemerella anatipestifer by ERIC-PCR and correlation with serotypes. Avian Pathol. 2019;48(1):12–6.

    Article  CAS  PubMed  Google Scholar 

  20. CLSI. M100 Performance Standars for Antimicrobial Susceptibility Testing, 29th ed. Clinical and Laboratory Standards Institute:Wayne, PA, USA, 2019.

  21. Ploy MC, Giamarellou H, Bourlioux P, Courvalin P, Lambert T, Detection. of aac(6’)-I genes in amikacin-resistant Acinetobacter spp. by PCR. Antimicrobial Agents and Chemotherapy 1994, 38(12):2925–2928.

  22. Ng LK, Martin I, Alfa M, Mulvey M. Multiplex PCR for the detection of Tetracycline resistant genes. Mol Cell Probes. 2001;15(4):209–15.

    Article  CAS  PubMed  Google Scholar 

  23. Kerrn MB. Susceptibility of Danish Escherichia coli strains isolated from urinary tract infections and bacteraemia, and distribution of Sul genes conferring Sulphonamide resistance. J Antimicrob Chemother. 2002;50(4):513–6.

    Article  CAS  PubMed  Google Scholar 

  24. Gordon L, Cloeckaert A, Doublet B, Schwarz S, Bouju-Albert A, Ganiere JP, Le Bris H, Le Fleche-Mateos A, Giraud E. Complete sequence of the floR-carrying multiresistance plasmid pAB5S9 from freshwater Aeromonas bestiarum. J Antimicrob Chemother. 2008;62(1):65–71.

    Article  CAS  PubMed  Google Scholar 

  25. Egan S, Barbosa AD, Feng Y, Xiao L, Ryan U. The risk of wild birds contaminating source water with zoonotic Cryptosporidium and Giardia is probably overestimated. Sci Total Environ. 2024;912:169032.

    Article  CAS  PubMed  Google Scholar 

  26. Ke Y, Han X, Si-Ru Lin S, Wu H, Li Y, Liu R, Liao M, Jia W. Emergence of a triple reassortment avian influenza virus (A/H5N6) from wild birds. J Infect. 2024;88(3):106106.

    Article  CAS  PubMed  Google Scholar 

  27. Xi Y, Wood C, Lu B, Zhang Y. Prevalence of a septicemia disease in the crested ibis (Nipponia nippon) in China. Avian Dis. 2007;51(2):614–7.

    Article  PubMed  Google Scholar 

  28. Chen S, Hao H, Liu Q, Wang R, Zhang P, Wang X, Du E, Yang Z. Phylogenetic and pathogenic analyses of two virulent Newcastle disease viruses isolated from crested Ibis (Nipponia nippon) in China. Virus Genes. 2013;46(3):447–53.

    Article  CAS  PubMed  Google Scholar 

  29. Li Z, Liu P, Hou J, Xu G, Zhang J, Lei Y, Lou Z, Liang L, Wen Y, Zhou J. Detection of Chlamydia psittaci and Chlamydia Ibidis in the endangered crested Ibis (Nipponia nippon). Epidemiol Infect. 2020;148:e1.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Cavaliere AF, Perelli F, Mattei A, Poggetto PD, Marchi L, Vidiri A, Turrini I, Aquilini D, Brunelli T, Scambia G, et al. Case report: vertical transmission of Plesiomonas shigelloides. Is it time to strengthen information on safety concerns for Raw seafood dietary exposure in pregnancy? J Maternal-Fetal Neonatal Med. 2023;36(1):2220061.

    Article  Google Scholar 

  31. Murray CJL, Ikuta KS, Sharara F, Swetschinski L, Robles Aguilar G, Gray A, Han C, Bisignano C, Rao P, Wool E, et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet. 2022;399(10325):629–55.

    Article  CAS  Google Scholar 

  32. Akhter S, Bhat MA, Ahmed S, Siddiqui WA. Antibiotic residue contamination in the aquatic environment, sources and associated potential health risks. Environ Geochem Health 2024, 46(10).

  33. Holmberg SD, Wachsmuth IK, Hickman-Brenner FW, Blake PA. Farmer 3rd JJ: Plesiomonas enteric infections in the united States. Ann Intern Med. 1984;105(5):690–4.

    Article  Google Scholar 

  34. Bravo FL, Correa MY, CIJ F, Fernández AA, Ramírez AM, Núñez FF, Ledo GY, Cruz IY. Virulence factors and in vitro susceptibility of Plesiomonas shigelloides isolated from diarrhea episodes in Cuba. Revista Chil De Infectologia. 2009;26(3):233–8.

    Google Scholar 

  35. Selim S, Almuhayawi MS, Zakai SA, Salama AA. Distinction between antimicrobial resistance and putative virulence genes characterization in Plesiomonas shigelloides isolated from different sources. Antibiot (Basel). 2022;11(1):85.

    Article  CAS  Google Scholar 

  36. Avison MB, Bennett PM, Walsh TR. β-Lactamase expression in Plesiomonas shigelloides. J Antimicrob Chemother. 2000;45(6):877–80.

    Article  CAS  PubMed  Google Scholar 

  37. Wong T, Tsui H, So M, Lai J, Lai S, Tse C, Ng T. Plesiomonas shigelloides infection in Hong Kong: retrospective study of 167 laboratory-confirmed cases. Hong Kong Med J. 2000;6(4):375–80.

    CAS  PubMed  Google Scholar 

  38. Kabir F, Zhang P, Yao H, Ji L, Chen L, Xu D, Yan W. Pathogenic characteristics of an aggregated diarrhea event caused by Plesiomonas shigelloides from stream. PLoS ONE 2024, 19(4).

  39. Hostacká A, Ciznár I. Some properties of Plesiomonas shigelloides treated with aminoglycosides. Folia Microbiol. 2003;48(5):659–63.

    Article  Google Scholar 

  40. Plattner M, Catelani M, Gmür S-L, Hartmann M, Kiliç F, Haldimann K, Crich D, Hobbie SN. Phenotypic differentiation within the aac(6′) aminoglycoside resistance gene family suggests a novel subtype IV of contemporary clinical relevance. Antibiotics 2024, 13(12).

  41. Robicsek A, Strahilevitz J, Jacoby GA, Macielag M, Abbanat D, Hye Park C, Bush K, Hooper DC. Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. Nat Med. 2005;12(1):83–8.

    Article  PubMed  Google Scholar 

  42. Xie Z, Jian J, Chen L. Epidemiology and mechanism of drug resistance of Multidrug-Resistant Klebsiella Pneumoniae isolated from patients with urinary tract infection in Beijing teaching hospital, China. Infect Drug Resist. 2025;18:135–49.

    Article  PubMed  PubMed Central  Google Scholar 

  43. Basnet A, Chand AB, Bajracharya S, Shrestha MR, Shrestha S, Tamang B, Dulal M, Pokhrel N, Shrestha LB. Biofilm formation and Plasmid-Mediated quinolone resistance genes at varying quinolone inhibitory concentrations in quinolone-Resistant Bacteria superinfecting COVID-19 inpatients. Am J Trop Med Hyg. 2025;112(2):346–54.

    Article  CAS  PubMed  Google Scholar 

  44. Ke T, Yang D, Yan Z, Yin L, Shen H, Luo C, Xu J, Zhou Q, Wei X, Chen F. Identification and pathogenicity analysis of the pathogen causing spotted spleen in muscovy Duck. Front Veterinary Sci 2022, 9.

  45. Stock I, Wiedemann B. Natural antimicrobial susceptibilities of Plesiomonas shigelloides strains. J Antimicrob Chemother. 2001;48(6):803–11.

    Article  CAS  PubMed  Google Scholar 

  46. Meads MB, Gatenby RA, Dalton WS. Environment-mediated drug resistance: a major contributor to minimal residual disease. Nat Rev Cancer. 2009;9(9):665–74.

    Article  CAS  PubMed  Google Scholar 

  47. Peng J-J, Balasubramanian B, Ming Y-Y, Niu J-L, Yi C-M, Ma Y, Liu W-C. Identification of antimicrobial resistance genes and drug resistance analysis of Escherichia coli in the animal farm environment. J Infect Public Health. 2021;14(12):1788–95.

    Article  PubMed  Google Scholar 

  48. Collineau L, Bourély C, Rousset L, Berger-Carbonne A, Ploy M-C, Pulcini C, Colomb-Cotinat M. Towards One Health surveillance of antibiotic resistance: characterisation and mapping of existing programmes in humans, animals, food and the environment in France, 2021. Eurosurveillance 2023, 28(22).

  49. Tsugawa H, Ogawa A, Takehara S, Kimura M, Okawa Y. Primary structure and function of a cytotoxic outer-membrane protein (ComP) of Plesiomonas shigelloides. FEMS Microbiol Lett. 2008;281(1):10–6.

    Article  CAS  PubMed  Google Scholar 

  50. Yan J, Zhang Z, Shi H, Xue X, Li A, Ding P, Guo X, Wang J, Wang Y, Cao B. Transcriptome analysis reveals Cross-Talk between the flagellar transcriptional hierarchy and secretion system in Plesiomonas shigelloides. Int J Mol Sci. 2024;25(13):7375.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Baratéla KC, Saridakis HO, Gaziri LC, Pelayo JS. Effects of medium composition, calcium, iron and oxygen on haemolysin production by Plesiomonas shigelloides isolated from water. J Appl Microbiol. 2001;90(3):482–7.

    Article  PubMed  Google Scholar 

  52. Ekundayo TC, Okoh AI. Pathogenomics of virulence traits of Plesiomonas shigelloides that were deemed inconclusive by traditional experimental approaches. Front Microbiol 2018, 9.

  53. Yin Z, Yuan C, Zhang S, Wei Y, Jiang L, Wang M, Ma S, Du Y. Horizontal gene transfer clarifies taxonomic confusion and promotes the genetic diversity and pathogenicity of Plesiomonas shigelloides. mSystems. 2020;5(5):e00448–00420.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

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Funding

This work was supported by the National Key Research and Development Program of China (2023YFD1801000), Zhejiang A&F University Talents Starting Program (2024LFR132), and the school enterprise cooperation project (H20200422).

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Author notes

  1. Jing Xia and Lele Shao contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Applied Biotechnology on Animal Science & Veterinary Medicine of Zhejiang Province, Zhejiang Engineering Research Center for Veterinary Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin’an District, Hangzhou, Zhejiang Province, 311300, China

    Jing Xia, Lele Shao, Xuanyi Chen, Yi Ji, Wulin Ma, Keyuan Chen, Houhui Song & Yongchun Yang

  2. Deqing County Ecological Forestry Comprehensive Service Center, Deqing, Zhejiang Province, 313200, China

    Guoqiang Qiu

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Contributions

This study was conceived and designed by YY, HS, and GQ. The experiments were performed mainly by LS, with the assistance of XC, YJ, WM, and KC. JX and LS analyzed the data. JX wrote the original manuscript, with the manuscript modified by GQ, HS, and YY. All authors reviewed the manuscript.

Corresponding authors

Correspondence to Houhui Song or Yongchun Yang.

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Ethics approval and consent to participate

All experiments were conducted in accordance with the guidelines and regulations outlined by the Management and Use of Laboratory Animals of Zhejiang Province and complied with China’s existing laws and regulations for biological research. We only collected feces from the cages of the crested ibises and did not perform any procedures on these birds, minimizing the impact on them. The animals’ experiments were conducted in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals approved by the State Council of the People’s Republic of China. The protocol received approval from the Institutional Animal Care and Use Committee of Zhejiang A&F University (Permit Number: ZAFUAC2023001).

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The authors declare no competing interests.

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Xia, J., Shao, L., Chen, X. et al. First report of multidrug-resistant and pathogenic Plesiomonas shigelloides from endangered crested ibis (Nipponia nippon). BMC Vet Res 21, 299 (2025). https://doiorg.publicaciones.saludcastillayleon.es/10.1186/s12917-025-04755-3

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BMC Veterinary Research

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